PURIFICATION AND CHARACTERIZATION OF A (1,4)-BETA-D-ARABINOXYLAN ARABINOFURANOHYDROLASE FROM ASPERGILLUS-AWAMORI

被引：0
|
作者：
KORMELINK, FJM
SEARLEVANLEEUWEN, MJF
WOOD, TM
VORAGEN, AGJ
机构：
[1] AGR UNIV WAGENINGEN,BIOTECHNION,DEPT FOOD SCI,BOMENWEG 2,6703 HD WAGENINGEN,NETHERLANDS
[2] ROWETT RES INST,BUCKSBURN AB2 9SB,ABERDEEN,SCOTLAND
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 35卷 / 06期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An enzyme able to split off arabinose side-chains from cereal arabinoxylans was isolated from a cell-free culture filtrate of Aspergillus awamori CMI 142717 containing milled oat straw as the carbon source. The enzyme was highly specific for arabinoxylans and, unlike other alpha-L-arabinofuranosidases reported in the literature, did not show any activity towards p-nitrophenyl alpha-L-arabinofuranoside, arabinans and arabinogalactans. This novel enzyme, which can be described as a (1,4)-beta-D-arabinoxylan arabinofuranohydrolase, had a molecular mass of 32 000 Da when determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and a specific activity of 22 units/mg on wheat arabinoxylan.
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页码：753 / 758
页数：6
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