MOLECULAR KARYOTYPE OF ENTAMOEBA-HISTOLYTICA AND ENTAMOEBA-INVADENS

被引：18
|
作者：
VALDES, J [1 ]
HERNANDEZ, FD [1 ]
OCADIZ, R [1 ]
OROZCO, E [1 ]
机构：
[1] INST POLITECN NACL, CINVESTAV, DEPT GENET & MOLEC BIOL, APDO POSTAL 14-740, MEXICO CITY 07000, DF, MEXICO
来源：
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1990年 / 84卷 / 04期
关键词：
D O I：
10.1016/0035-9203(90)90032-A
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
We report the size-fractionation of Entamoeba histolytica and E. invadens deoxyribonucleic acid (DNA) by pulsed field gradient electrophoresis. Using 3 different electrophoretic conditions, we were able to resolve 6 to 9 bands between 300 and 2000 kilobases (kb), distributed in 16-22 large and up to 31 small chromosomes for E. histolytica DNA. For E. invadens, 4 to 5 bands between 300 and over 2000 kb were resolved and discriminated in 4 large and 6 small chromosomes. A ribosomal probe from Trypanosoma brucei hybridized with a 1100 kb band in E. histolytica strain HM1:IMSS and with a 1000 kb band in clone A of strain HM1:IMSS. Both cell lines also showed hybridization at the origin. The ribosomal probe hybridized only at the origin of the E. invadens DNA lane. A triosephosphate isomerase DNA probe from T. brucei hybridized only with a 2000 kb band in E. invadens, indicating that banding patterns are chromosomal bands and ruling out the possibility of DNA degradation. © 1990, Royal Society of Tropical Medicine and Hygiene. All rights reserved.
引用
收藏
页码：537 / 541
页数：5
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