CALCIUM INDICATORS AND EXCITOTOXICITY IN CULTURED CORTICAL-NEURONS

被引：0

作者：

AHERN, KV ^{[1
]}

LUSTIG, HS ^{[1
]}

CHAN, J ^{[1
]}

GREENBERG, DA ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO, SAN FRANCISCO GEN HOSP, DEPT NEUROL, SAN FRANCISCO, CA 94110 USA

来源：

NEUROSCIENCE LETTERS | 1993年 / 162卷 / 1-2期

关键词：

CALCIUM; EXCITOTOXICITY; FLUO-3; FURA-2; LACTATE DEHYDROGENASE; NMDA; PRIMARY NEURONAL CULTURE;

D O I：

暂无

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Membrane-permeating, fluorescent Ca2+ indicators have been used to investigate the role of increased intracelluLar Ca2+ (Ca2+i) levels in excitotoxic neuronal injury, but their ability to chelate Ca2+i and their own toxic effects in some cells could obscure this relationship. N-Methyl-D-aspartate (NMDA)-stimulated Ca2+i responses and toxicity were measured in neuron-enriched rat cerebrocortical cultures loaded with either fluo-3 or fura-2. Ca2+ responses signaled by both indicators were similar in magnitude, and neither indicator reduced NMDA toxicity, measured by lactate dehydrogenase (LDH) release. Fluo-3 and fura-2 appear to be suitable for comparative studies of NMDA-induced Ca2+i responses and excitotoxicity.

引用

页码：169 / 172

页数：4

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