CALCIUM INDICATORS AND EXCITOTOXICITY IN CULTURED CORTICAL-NEURONS

被引:0
|
作者
AHERN, KV [1 ]
LUSTIG, HS [1 ]
CHAN, J [1 ]
GREENBERG, DA [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO, SAN FRANCISCO GEN HOSP, DEPT NEUROL, SAN FRANCISCO, CA 94110 USA
关键词
CALCIUM; EXCITOTOXICITY; FLUO-3; FURA-2; LACTATE DEHYDROGENASE; NMDA; PRIMARY NEURONAL CULTURE;
D O I
暂无
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Membrane-permeating, fluorescent Ca2+ indicators have been used to investigate the role of increased intracelluLar Ca2+ (Ca2+i) levels in excitotoxic neuronal injury, but their ability to chelate Ca2+i and their own toxic effects in some cells could obscure this relationship. N-Methyl-D-aspartate (NMDA)-stimulated Ca2+i responses and toxicity were measured in neuron-enriched rat cerebrocortical cultures loaded with either fluo-3 or fura-2. Ca2+ responses signaled by both indicators were similar in magnitude, and neither indicator reduced NMDA toxicity, measured by lactate dehydrogenase (LDH) release. Fluo-3 and fura-2 appear to be suitable for comparative studies of NMDA-induced Ca2+i responses and excitotoxicity.
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页码:169 / 172
页数:4
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