GENE SEQUENCE OF RECA(+) AND CONSTRUCTION OF RECA MUTANTS OF VIBRIO-CHOLERAE

被引：10
|
作者：
STROEHER, UH [1 ]
LECH, AJ [1 ]
MANNING, PA [1 ]
机构：
[1] UNIV ADELAIDE,DEPT MICROBIOL & IMMUNOL,MICROBIAL PATHOGENESIS UNIT,ADELAIDE,SA 5005,AUSTRALIA
来源：
MOLECULAR & GENERAL GENETICS | 1994年 / 244卷 / 03期
关键词：
DNA REPAIR; RECOMBINATION; VIRULENCE; MUTATION;
D O I：
10.1007/BF00285457
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The recA(+) gene of Vibrio cholerae O1 has been cloned, its nucleotide sequence determined and the product characterized. A deletion mutation was constructed in the recA gene and mutants showed the typical sensitivity to UV and to DNA-damaging agents, as well as an inability to mediate homologous DNA recombination. The chromosomal recA deletion mutants in V. cholerae do not show altered virulence in the infant mouse cholera model and are thus ideal strains for use in complementation studies.
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页码：295 / 302
页数：8
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