A COMPARISON OF POLYMERASE CHAIN-REACTION AND AN INFECTIVITY ASSAY FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TITRATION DURING VIRUS INACTIVATION OF BLOOD COMPONENTS

被引:12
|
作者
HART, H
MCOMISH, F
HART, WG
SIMMONDS, P
YAP, PL
机构
[1] ROYAL EDINBURGH & ASSOCIATED HOSP,SE SCOTLAND BLOOD TRANSFUS SERV,EDINBURGH,SCOTLAND
[2] UNIV EDINBURGH,SCH MED,DEPT MED MICROBIOL,EDINBURGH EH8 9YL,MIDLOTHIAN,SCOTLAND
关键词
D O I
10.1046/j.1537-2995.1993.331094054622.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Three examples of human plasma-derived concentrates, intermediate-purity factors VIII and IX, and fibrinogen were spiked with tissue culture-grown human immunodeficiency virus type 1 (HIV-1) strain RF. All examples were freeze-dried and heated at 80-degrees-C for 72 hours by using validated production process models. HIV-1 infectivity was measured by a syncytial infectivity assay in C8166 cells and then compared with levels determined by nested HIV polymerase chain reaction (PCR). The infectivity assay demonstrated a reduction index of at least 4.5 log10, while PCR showed an average 1.7 log10. Large amounts of HIV-1 RNA (10(5)) were still detectable by PCR in samples in which infectivity assays failed to detect any HIV-1. These data suggest that HIV-1 PCR levels do not parallel HIV-1 infectivity levels during virus-inactivation procedures involved in coagulation factor concentrate production. PCR was able to detect the RNA associated with inactivated HIV-1 particles in the factor concentrates, which allows the conclusion that PCR is not a useful test with which to monitor virus-inactivation procedures such as heating at 80-degrees-C for 72 hours. This judgment contrasts with the more definite and sensitive role of PCR in diagnosing HIV-1 infection in patients in whom a positive HIV-1 PCR result correlates with active HIV-1 infection and with PCR's usefulness in monitoring virus removal.
引用
收藏
页码:838 / 841
页数:4
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