EXPERIMENTS, CORRELATION AND PREDICTION OF PROTEIN PARTITION-COEFFICIENT IN AQUEOUS 2-PHASE SYSTEMS CONTAINING PEG AND K2HPO4-KH2PO4

被引：30
|
作者：
PENG, QH [1 ]
LI, ZC [1 ]
LI, YG [1 ]
机构：
[1] TSING HUA UNIV,DEPT CHEM ENGN,BEIJING 100084,PEOPLES R CHINA
来源：
FLUID PHASE EQUILIBRIA | 1995年 / 107卷 / 02期
基金：
中国国家自然科学基金;
关键词：
EXPERIMENTS; DATA; METHOD; LIQUID-LIQUID EQUILIBRIA; LATTICE MODEL; PARTITION COEFFICIENT; ELECTROLYTE; POLYMER; PROTEIN;
D O I：
10.1016/0378-3812(94)02674-P
中图分类号：
O414.1 [热力学];
学科分类号：
摘要：
A quantitative analytical method for the study of the liquid-liquid equilibria (LLE) of K2HPO4-KH2PO4-PEG-protein aqueous two-phases systems has been established. 23 partition coefficients for six proteins at 25 degrees C were measured. The Baskir adsorption lattice model and Fitter long-range electrostatic term were extended to calculate the protein partition coefficients in aqueous two-phase systems containing electrolyte and polymer. Two adsorption energy parameters were regressed from six sets of experimental data of lysozyme-containing systems and can predict the other 17 partition coefficients of lysozyme and five other proteins in aqueous two-phase systems. The average relative deviation of the partition coefficient between experiment and calculation is less than 6%. The factors affecting protein partition coefficient were discussed.
引用
收藏
页码：303 / 315
页数：13
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