A SIMPLE METHOD FOR ISOLATION OF DNA FRAGMENTS ASSOCIATED WITH THE NUCLEAR LAMINA INVIVO

被引：2

作者：

CHRISTOVA, R

GALCHEVAGARGOVA, Z

机构：

[1] Institute of Molecular Biology, Bulgarian Academy of Sciences

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 20卷 / 02期

关键词：

Lamina-DNA purification; Nuclear lamina; u.v.-Crosslinking;

D O I：

10.1016/0165-022X(90)90069-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple method for the purification of DNA fragments associated with the nuclear lamina in vivo. Ehrlich ascite tumor cells are first u.v.-irradiated to crosslink DNA to proteins. The nuclear lamina is then isolated and purified by low-speed centrifugation through a cushion of 40% sucrose. The material sedimenting through the created density barrier represents nuclear lamina of a very high purity, free from any DNA fragments except those which were in a crosslinking distance to it in vivo. © 1990.

引用

页码：107 / 111

页数：5

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