NITRATE UPTAKE KINETICS BY 2 MARINE DIATOMS USING THE RADIOACTIVE-TRACER N-13

被引：34
|
作者：
COLLOS, Y
SIDDIQI, MY
WANG, MY
GLASS, ADM
HARRISON, PJ
机构：
[1] IFREMER, CNRS, CTR RECH ECOL MARINE & AQUACULTURE, CNRS, HOUMGAU, FRANCE
[2] UNIV BRITISH COLUMBIA, DEPT BOT, VANCOUVER V6T 1W5, BC, CANADA
[3] UNIV BRITISH COLUMBIA, DEPT OCEANOG, VANCOUVER V6T 1W5, BC, CANADA
来源：
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY | 1992年 / 163卷 / 02期
关键词：
EUTROPHIC AREA; NITRATE UPTAKE KINETICS; N-13; LABEL;
D O I：
10.1016/0022-0981(92)90053-D
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
The response of the nitrate uptake system to nitrate concentrations in the range 1 to 500 muM was examined with the radioactive isotope N-13 for two marine diatoms (Skeletonema costatum and Thalassiosira weissflogii) under various states of nitrogenous nutrition. Classical saturation kinetics were observed in the lower range of concentrations (1-40 muM), but a further increase in uptake was observed above 60 muM. Intracellular nitrate concentrations between 5 and 17 mM indicate that it is an active uptake system rather than a diffusion phenomenon which is responsible for uptake at those high external nitrate levels.
引用
收藏
页码：251 / 260
页数：10
相关论文
共 50 条
  • [41] NITROGEN AND CARBON ASSIMILATION IN N2-FIXING PLANTS - SHORT-TERM STUDIES USING N-13 AND C-11
    SCHUBERT, KR
    COKER, GT
    ADVANCES IN CHEMISTRY SERIES, 1981, (197): : 317 - 339
  • [42] C-13 AND N-15 UPTAKE BY MARINE-PHYTOPLANKTON .4. UPTAKE RATIOS AND THE CONTRIBUTION OF NITRATE TO THE PRODUCTIVITY OF ANTARCTIC WATERS (INDIAN-OCEAN SECTOR)
    COLLOS, Y
    SLAWYK, G
    DEEP-SEA RESEARCH PART A-OCEANOGRAPHIC RESEARCH PAPERS, 1986, 33 (08): : 1039 - 1051
  • [43] The Influence of Ammonium on Nitrate Uptake and Assimilation in 2-Year-Old Ash and Oak Trees - A Tracer-Study with 15N
    STADLER, J
    GEBAUER, G
    SCHULZE, ED
    ISOTOPENPRAXIS, 1993, 29 (1-2): : 85 - 92
  • [44] AEROBIC EMISSIONS OF N2O AND N-2 FROM SOIL CORES - MEASUREMENT PROCEDURES USING N-13 LABELED NO3- AND NH4+
    SPEIR, TW
    KETTLES, HA
    MORE, RD
    SOIL BIOLOGY & BIOCHEMISTRY, 1995, 27 (10): : 1289 - 1298
  • [45] STUDIES ON DEPOSITION OF INHALED FIBROUS MATERIAL IN RESPIRATORY-TRACT OF RAT AND ITS SUBSEQUENT CLEARANCE USING RADIOACTIVE-TRACER TECHNIQUES .2. DEPOSITION OF UICC STANDARD REFERENCE SAMPLES OF ASBESTOS
    MORGAN, A
    EVANS, JC
    EVANS, RJ
    HOUNAM, RF
    HOLMES, A
    DOYLE, SG
    ENVIRONMENTAL RESEARCH, 1975, 10 (02) : 196 - 207
  • [46] NOVEL BOLUS DOUBLE TRACER STUDY OF C-13(1) AND N-15(2) LYSINE KINETICS IN ADULT WOMEN
    THOMAS, MR
    IRVING, CS
    MALPHUS, EW
    GARZA, C
    MARKS, LM
    KLEIN, PD
    FEDERATION PROCEEDINGS, 1983, 42 (04) : 825 - 825
  • [47] AMMONIUM UPTAKE BY RICE ROOTS .2. KINETICS OF NH4+-N-13 INFLUX ACROSS THE PLASMALEMMA
    WANG, MY
    SIDDIQI, MY
    RUTH, TJ
    GLASS, ADM
    PLANT PHYSIOLOGY, 1993, 103 (04) : 1259 - 1267
  • [48] TRACER STUDIES WITH (NH4+)-N-13,(K+)-K-42,(MG2+)-MG-28 - A BUGS EYE VIEW OF THE PERIODIC TABLE
    SILVER, S
    PERRY, RD
    ADVANCES IN CHEMISTRY SERIES, 1981, (197): : 453 - 468
  • [49] DOUBLE (C-13-1, N-15-2) LYSINE TRACER METHOD FOR MULTICOMPARTMENTAL ANALYSIS OF LYSINE KINETICS IN ADULT WOMEN
    IRVING, CS
    THOMAS, MR
    MALPHUS, EW
    GARZA, C
    MARKS, LM
    KLEIN, PD
    CLINICAL RESEARCH, 1983, 31 (02): : A617 - A617
  • [50] DOUBLE (C-13-1,N-15-2) LYSINE TRACER METHOD FOR MULTICOMPARTMENTAL ANALYSIS OF LYSINE KINETICS IN ADULT WOMEN
    IRVING, CS
    THOMAS, MR
    MALPHUS, EW
    GARZA, C
    MARKS, LM
    KLEIN, PD
    AMERICAN JOURNAL OF CLINICAL NUTRITION, 1983, 37 (04): : 712 - 712
12345