FREEZE-THAW INJURY IS ENHANCED BY POSTTHAW LEACHING IN WATER

被引：0
|
作者：
ZHANG, MIN
WILLISON, JHM
XIAO, X
CHEUNG, CH
机构：
来源：
CANADIAN JOURNAL OF PLANT SCIENCE | 1994年 / 74卷 / 02期
关键词：
BRASSICA RAPA L; 2,3,5-TRIPHENYLTETRAZOLIUM CHLORIDE (TTC); FREEZE-THAW INJURY; LEACHING;
D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Turnip (Brassica rapa L.) root tissue was exposed to freeze-thaw stresses of -7, -9, -11 and -19 degrees C. The post-thawed tissues were either subjected to leaching in deionized water for 2 h or left at 100% humidity. Tissue survival was then assayed by vital staining using modified 2,3,5-triphenyltetrazolium chloride (TTC) staining in 0.2 M phosphate buffer. Tissue survival was significantly lower for leached samples than for non-leached samples. It is concluded that freeze-thaw injury in plant tissues is enhanced by post-thaw leaching in water. The 0.05 M phosphate buffer commonly used for TTC staining also damaged freeze-thaw injured tissue.
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收藏
页码：357 / 358
页数：2
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