CLONING OF A DNA PROBE AND ITS APPLICATION IN THE DETECTION OF PLASMODIUM-FALCIPARUM - A PRELIMINARY-REPORT

被引：0
|
作者：
LIU, KY
HUANG, BC
ZHANG, HH
LIU, YB
KONG, RQ
CHENG, YL
机构：
来源：
CHINESE MEDICAL JOURNAL | 1993年 / 106卷 / 01期
关键词：
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The clones containing parasite DNA fragments were screened from a genomic DNA library of Plasmodium falciparum FCC1 / HN isolate. A DNA probe derived from clone pBF4 consisting of 3 kilobase pairs hybridizes specifically with P. falciparum DNA but not with human DNA, P. cynomolgi DNA or P. berghei DNA. The nick translated radiolabelled probe can detect 10 pg purified P. falciparum DNA and a 0.001% parasitemia after 24 hours of film exposure. The probe reacts with all microscopically diagnosed P. falciparum samples and 3 of 41 P. vivax samples as well but not with any of 10 human DNA samples.
引用
收藏
页码：31 / 34
页数：4
相关论文
共 50 条
  • [21] DNA-SYNTHESIS IN GAMETOCYTES OF PLASMODIUM-FALCIPARUM
    JANSE, CJ
    PONNUDURAI, T
    LENSEN, AHW
    MEUWISSEN, JHET
    RAMESAR, J
    VANDERPLOEG, M
    OVERDULVE, JP
    PARASITOLOGY, 1988, 96 : 1 - 7
  • [22] PLASMODIUM-FALCIPARUM - EVIDENCE FOR A DNA METHYLATION PATTERN
    POLLACK, Y
    KOGAN, N
    GOLENSER, J
    EXPERIMENTAL PARASITOLOGY, 1991, 72 (04) : 339 - 344
  • [23] GENOME SIZE AND DNA COMPLEXITY OF PLASMODIUM-FALCIPARUM
    HOUGHEVANS, BR
    HOWARD, J
    BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 698 (01) : 56 - 61
  • [24] ANALYSIS OF CLINICAL SPECIMENS BY HYBRIDIZATION WITH PROBE CONTAINING REPETITIVE DNA FROM PLASMODIUM-FALCIPARUM
    FRANZEN, L
    WESTIN, G
    SHABO, R
    ASLUND, L
    PERLMANN, H
    PERSSON, T
    WIGZELL, H
    PETTERSSON, U
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1984, 20 (02) : 171 - 171
  • [25] INTERSPERSED REPETITIVE DNA FROM PLASMODIUM-FALCIPARUM
    WEBER, JL
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 29 (2-3) : 117 - 124
  • [26] DETECTION OF PLASMODIUM-FALCIPARUM ANTIGENS AND THEIR EXPRESSION INVITRO
    UPCROFT, JA
    MYLER, PJ
    KIDSON, C
    PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 1981, 14 : 99 - 99
  • [27] PCR DETECTION OF PLASMODIUM-FALCIPARUM BY OLIGONUCLEOTIDE PROBES
    JAUREGUIBERRY, G
    HATIN, I
    DAURIOL, L
    GALIBERT, G
    MOLECULAR AND CELLULAR PROBES, 1990, 4 (05) : 409 - 414
  • [28] DETECTION OF PLASMODIUM-FALCIPARUM DNA IN A MICROTITRATION PLATE-BASED HYBRIDIZATION TEST
    KORPELA, K
    LAAKSONEN, M
    KALLIO, A
    SODERLUND, H
    PETTERSSON, U
    KYRONSEPPA, H
    RANKI, M
    FEMS MICROBIOLOGY LETTERS, 1992, 90 (02) : 173 - 178
  • [29] CLONING OF A BETA-TUBULIN GENE FROM PLASMODIUM-FALCIPARUM
    DELVES, CJ
    RIDLEY, RG
    GOMAN, M
    HOLLOWAY, SP
    HYDE, JE
    SCAIFE, JG
    MOLECULAR MICROBIOLOGY, 1989, 3 (11) : 1511 - 1519
  • [30] CLONING OF ASEXUAL BLOOD-STAGE ANTIGENS OF PLASMODIUM-FALCIPARUM
    COPPEL, RL
    CREWTHER, PE
    FAVALORO, JM
    STAHL, HD
    PAPUA NEW GUINEA MEDICAL JOURNAL, 1986, 29 (01) : 75 - 80
12345