A SENSITIVE 2-SITE SANDWICH ENZYME-IMMUNOASSAY FOR HUMAN ANGIOTENSIN CONVERTING ENZYME UTILIZING MONOCLONAL-ANTIBODIES

被引：10
|
作者：
STEVENS, J
DANILOV, S
FANBURG, BL
LANZILLO, JJ
机构：
[1] NEW ENGLAND MED CTR,DIV PULM,750 WASHINGTON ST,BOX 19,BOSTON,MA 02111
[2] USSR CARDIOL RES CTR,INST EXPTL CARIOL,MOSCOW 121552,USSR
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 132卷 / 02期
关键词：
Angiotensin converting enzyme; Captopril; Enzyme immunoassay; Lisinopril;
D O I：
10.1016/0022-1759(90)90038-W
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive enzyme immunoassay was developed for human angiotensin converting enzyme. Monoclonal antibodies specific for two unique converting enzyme epitopes were utilized to develop a two-site sandwich enzyme immunoassay. Alkaline phosphatase conjugated to the detecting antibody hydrolyzes nicotinamide adenine dinucleotide phosphate (NADP) to NAD. Subsequently, NAD is cycled between its reduced and oxidized forms by an alcohol dehydrogenase/diaphorase catalyzed redox cycle. Each cycle converts iodonitrotetrazolium violet to a highly colored formazan which is quantitated. With this assay, as little as 94 pg/ml of native converting enzyme is detectable without interference from either therapeutic or endogenous converting enzyme inhibitors. © 1990.
引用
收藏
页码：263 / 273
页数：11
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