PERINATAL HYPOTHYROIDISM IMPAIRS THE NORMAL TRANSITION OF GLUT4 AND GLUT1 GLUCOSE TRANSPORTERS FROM FETAL TO NEONATAL LEVELS IN HEART AND BROWN ADIPOSE-TISSUE - EVIDENCE FOR TISSUE-SPECIFIC REGULATION OF GLUT4 EXPRESSION BY THYROID-HORMONE

被引:0
|
作者
CASTELLO, A
RODRIQUEZMANZANEQUE, JC
CAMPS, M
PEREZCASTILLO, A
TESTAR, X
PALACIN, M
SANTOS, A
ZORZANO, A
机构
[1] UNIV BARCELONA, FAC BIOL, DEPT BIOQUIM & FISIOL, E-08028 BARCELONA, SPAIN
[2] UNIV COMPLUTENSE MADRID, FAC MED, DEPT BIOQUIM & BIOL MOLEC, E-28040 MADRID, SPAIN
[3] CSIC, INST INVEST BIOMED, E-28029 MADRID, SPAIN
关键词
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
GLUT1 and GLUT4 glucose transporter expression is highly regulated in muscle and adipose tissue during perinatal life. Here we have investigated the role of thyroid hormones in the regulation of GLUT4 induction and GLUT1 repression associated to neonatal development. Perinatal hypothyroidism markedly impaired GLUT4 protein induction in heart. This effect was heart specific, and a greater expression of GLUT4 was detected in brown adipose tissue from neonatal hypothyroid rats compared with controls. These changes in GLUT4 protein expression were not detected in brown adipose tissue or heart when hypothyroidism was induced in adult rats. These results indicate that GLUT4 induction during perinatal life is highly sensitive to thyroid hormones in both heart and adipose tissue. Perinatal hypothyroidism was characterized by decreased cardiac GLUT4 mRNA concentrations. T-3 injection caused a marked increase in cardiac levels of GLUT4 mRNA in hypothyroid neonates. Thus, in 18-day-old hypothyroid rats, GLUT4 mRNA levels increased 3-fold 1 h after T-3 injection. Under these conditions, retinoic acid also caused a rapid increase in cardiac GLUT4 mRNA levels from hypothyroid neonates. In addition, cardiac levels of GLUT4 protein markedly increased in fetuses and in neonates 24 h after T-3 injection. These findings suggest that a direct effect of thyroid hormones is the promotion of cardiac GLUT4 gene expression. GLUT1 protein expression was markedly enhanced in brown adipose tissue and heart during neonatal hypothyroidism as well, as in hypothyroidism induced in adult rats. This was concomitant to greater levels of GLUT1 mRNA in hearts from hypothyroid neonates, Immunofluorescence analysis indicated that cardiomyocytes from hypothyroid pups contained an enhanced level of GLUT1 protein. Furthermore, T-3 injection caused a decrease in cardiac levels of GLUT1 mRNA in hypothyroid neonates. These results indicate that thyroid hormone manipulation leads to inverse regulation of GLUT1 and GLUT4 glucose transporter gene expression in the neonatal heart. We conclude that thyroid hormones play a pivotal role controlling the transition of glucose transporter carriers from fetal to neonatal levels in heart and brown adipose tissue.
引用
收藏
页码:5905 / 5912
页数:8
相关论文
共 26 条
  • [21] TIME-DEPENDENT REGULATION OF RAT ADIPOSE-TISSUE GLUCOSE TRANSPORTER (GLUT4) MESSENGER-RNA AND PROTEIN BY INSULIN IN STREPTOZOCIN-DIABETIC AND NORMAL RATS
    SIVITZ, WI
    DESAUTEL, SL
    LEE, EC
    PESSIN, JE
    METABOLISM-CLINICAL AND EXPERIMENTAL, 1992, 41 (11): : 1267 - 1272
  • [22] DEVELOPMENTAL REGULATION OF GLUT-1 (ERYTHROID HEP G2) AND GLUT-4 (MUSCLE FAT) GLUCOSE TRANSPORTER EXPRESSION IN RAT-HEART, SKELETAL-MUSCLE, AND BROWN ADIPOSE-TISSUE
    SANTALUCIA, T
    CAMPS, M
    CASTELLO, A
    MUNOZ, P
    NUEL, A
    TESTAR, X
    PALACIN, M
    ZORZANO, A
    ENDOCRINOLOGY, 1992, 130 (02) : 837 - 846
  • [23] GLUT4 MESSENGER-RNA CONCENTRATION IN WHITE ADIPOSE-TISSUE FROM RECENT ONSET TYPE-1 DIABETIC-PATIENTS
    EDDOUKS, M
    BURCELIN, R
    DELMAS, V
    GIRARD, J
    ASSAN, R
    DIABETOLOGIA, 1992, 35 : A90 - A90
  • [24] DIFFERENTIAL REGULATION OF ADIPOSE-TISSUE GLUCOSE TRANSPORTERS IN GENETIC OBESITY (FATTY RAT) - SELECTIVE INCREASE IN THE ADIPOSE CELL MUSCLE GLUCOSE TRANSPORTER (GLUT-4) EXPRESSION
    HAINAULT, I
    GUERREMILLO, M
    GUICHARD, C
    LAVAU, M
    JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (03): : 1127 - 1131
  • [25] Age-related adipose tissue mRNA expression of ADD1/SREBP1, PPARγ, lipoprotein lipase, and GLUT4 glucose transporter in rhesus monkeys
    Hotta, K
    Bodkin, NL
    Gustafson, TA
    Yoshioka, S
    Ortmeyer, HK
    Hansen, BC
    JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 1999, 54 (05): : B183 - B188
  • [26] Hyperbaric Oxygen Ameliorates Insulin Sensitivity by Increasing GLUT4 Expression in Skeletal Muscle and Stimulating UCP1 in Brown Adipose Tissue in T2DM Mice
    Liu, Yuan
    Zhang, Di
    Yuan, Junhua
    Song, Limin
    Zhang, Caishun
    Lin, Qian
    Li, Manwen
    Sheng, Zhi
    Ma, Zhengye
    Lv, Fengyuan
    Gao, Guangkai
    Dong, Jing
    FRONTIERS IN ENDOCRINOLOGY, 2020, 11