ANTISERUM TO CARCINOEMBRYONIC ANTIGEN RECOGNIZES A PHOSPHOTYROSINE-CONTAINING PROTEIN IN HUMAN COLON CANCER CELL-LINES

被引：21

作者：

SKUBITZ, KM

DUCKER, TP

SKUBITZ, APN

GOUELI, SA

机构：

[1] UNIV MINNESOTA,SCH MED,DEPT MED,MINNEAPOLIS,MN 55455

[2] UNIV MINNESOTA,SCH MED,DEPT LAB MED & PATHOL,MINNEAPOLIS,MN 55455

[3] UNIV MINNESOTA,SCH MED,CTR BIOMED ENGN,MINNEAPOLIS,MN 55455

[4] MASONIC CANC CTR,MINNEAPOLIS,MN 55455

[5] VET ADM MED CTR,MINNEAPOLIS,MN 55417

来源：

FEBS LETTERS | 1993年 / 318卷 / 02期

关键词：

BILIARY GLYCOPROTEIN; CARCINOEMBRYONIC ANTIGEN; ECTOPROTEIN KINASE; PROTEIN PHOSPHORYLATION; PHOSPHOTYROSINE;

D O I：

10.1016/0014-5793(93)80021-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Members of the carcinoembryonic antigen (CEA) family include CEA, non-specific cross reacting antigen (NCA), and biliary glycoprotein (BGP), and appear to function as cell adhesion molecules. Immunoprecipitation and subsequent gel electrophoresis of proteins from several colon cancer cell lines labeled with [gamma-P-32]ATP, under conditions designed to detect ecto-kinase-catalyzed phosphorylation of cellular proteins, revealed that polyclonal anti-CEA antiserum recognized a 175-190 kDa phosphoprotein on the surface of colon cancer cells. The ability to detect this phosphoprotein did not correlate with CEA production, and immunoprecipitation studies suggested that the phosphoprotein is BGP. Phosphoamino acid analysis of the 175-190 kDa protein showed that it contained predominantly phosphotyrosine.

引用

页码：200 / 204

页数：5

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