PARTIAL ISOLATION AND SOME PROPERTIES OF ENTEROTOXIN PRODUCED BY BACILLUS-CEREUS STRAINS

被引：3
|
作者：
GUAYCURUS, TV
VICENTE, AC
DESIMONE, SG
RABINOVITCH, L
机构：
[1] INST OSWALDO CRUZ,DEPT BACTERIOL,AV BRAZIL 4365,BR-21045 RIO JANEIRO,BRAZIL
[2] INST OSWALDO CRUZ,DEPT GENET,BR-21045 RIO JANEIRO,BRAZIL
[3] INST OSWALDO CRUZ,DEPT BIOQUIM & BIOL MOLEC,BR-21045 RIO JANEIRO,BRAZIL
[4] UFF,DEPT BIOL CELULAR & MOLEC,NITEROI,RJ,BRAZIL
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1993年 / 88卷 / 01期
关键词：
BACILUS-CEREUS; ENTEROTOXIN; TOXIC FACTOR; PURIFICATION;
D O I：
10.1590/S0074-02761993000100020
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Vero and Hela cells. The analysis by SDS-PAGE after ultrafiltration confirm recent findings that the enterotoxin is a compound with molecular mass > 30.000.
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页码：131 / 134
页数：4
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