A SIMPLIFIED HPLC METHOD FOR THE DETERMINATION OF PHYTOESTROGENS IN SOYBEAN AND ITS PROCESSED PRODUCTS

被引：144

作者：

WANG, GJ ^{[1
]}

KUAN, SS ^{[1
]}

FRANCIS, OJ ^{[1
]}

WARE, GM ^{[1
]}

CARMAN, AS ^{[1
]}

机构：

[1] US FDA, NAT TOXINS RES CTR, 4298 ELYSIAN FIELDS AVE, NEW ORLEANS, LA 70122 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1990年 / 38卷 / 01期

关键词：

D O I：

10.1021/jf00091a041

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

By using the proposed procedure, phytoestrogens (daidzein, genistein, coumestrol) can be isolated from soybean and its processed products and subsequently quantitated by HPLC without defatting and cleanup of the samples prior to assay. The samples are extracted with acetonitrile-water, and the extract is filtered through a glass fiber filter. The analytes in the filtrate are in turn separated by HPLC on a C18 column and quantified by spectrometry. The method is sensitive to 2 ppm of isoflavones with UV detection and 0. 5 ppm of coumestrol with fluorescent detection. The recoveries of phytoestrogens in spiked samples ranged between 75 and 110%. The rapidity, simplicity, and low cost of the method make feasible the assay of large numbers of samples in a regulatory laboratory. © 1990, American Chemical Society. All rights reserved.

引用

页码：185 / 190

页数：6

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