CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDY OF MINOR GLUCOAMYLASE FROM ASPERGILLUS-AWAMORI VARIANT-X-100/D27

被引：12
|
作者：
GOLUBEV, AM
NEUSTROEV, KN
ALESHIN, AE
FIRSOV, LM
机构：
[1] Leningrad Nuclear Physics Institute U.S.S.R. Academy of Sciences Gatchina, Leningrad district
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 226卷 / 01期
关键词：
CRYSTALLIZATION; GLUCOAMYLASE; X-RAY;
D O I：
10.1016/0022-2836(92)90139-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with α-mannosidase to partly remove the sugar component. The crystals belong to the space group P212121 with cell dimensions a = 116.7 A ̊, b = 104.3 A ̊, c = 48.5 A ̊ and diffract beyond 2.5 Å resolution. © 1992.
引用
收藏
页码：271 / 272
页数：2
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