SIMULTANEOUS DETERMINATION OF TINIDAZOLE AND FURAZOLIDONE IN SUSPENSION BY HPTLC AND HPLC

High performance thin layer chromatographic (HPTLC) and high performance liquid chromatographic (HPLC) methods were developed for the simultaneous determination of Tinidazole and Furazolidone in suspension. In the HPTLC method the separation of Tinidazole and Furazolidone was carried out on silica gel 60F(254) HPTLC glass plate using chloroform: methanol: ammonia (9:1:0.1 v/v) as a mobile phase. Rf values obtained were 0.63 and 0.79 for Furazolidone and Tinidazole respectively. Densitometric evaluation was done at 335 nm. Linearity was obtained within the concentration range 10-50 mu g/ml and 3.5-17.5 mu g/ml for Tinidazole and Furazolidone respectively. The second method is based on high performance liquid chromatography on a reversed phase column (mu Bondapak C-18) using a mobile phase comprised of water : acetonitrile : triethylamine (80:20:0.1 v/v) adjusted to pH = 3.0 with dil. phosphoric acid. Retention times were 5.24 and 7.82 min for Tinidazole and Furazolidone respectively at a flow rate of 1.5 ml/min. Detection was done at 335 nm. Linearity was obtained within the concentration range 30-180 mu g/ml and 10.5-63 mu g/ml for TinidazoIe and Furazolidone resp.