NEW AFFINITY SORBENTS FOR PURIFICATION OF RECOMBINANT PROTEINS WITH THE USE OF CHITIN-BINDING DOMAIN AS AN AFFINITY TAG

被引：0
|
作者：
Kurek, Denis, V [1 ]
Lopatin, Sergey A. [1 ]
Tikhonov, Vladimir E. [2 ]
Varlamov, Valery P. [1 ]
机构：
[1] RAS, Ctr Bioengn, Moscow, Russia
[2] RAS, AN Nesmeyanov Inst Organoelement Cpds, Moscow, Russia
来源：
PROGRESS ON CHEMISTRY AND APPLICATION OF CHITIN AND ITS DERIVATIVES | 2010年 / 15卷
关键词：
chitin based sorbents; affinity chromatography; chitin-binding domain;
D O I：
暂无
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
With the increasing amounts of recombinant proteins production the problem of effective purification of produced proteins became one of major topics for research nowadays. Affinity chromatography proved to be the most dominant method for this aim and application of chitin based affinity sorbents in combination with use of chitin-binding domain as an affinity tag for purification of recombinant proteins showed its efficiency even in comparison with commercially available systems.
引用
收藏
页码：41 / 46
页数：6
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