NEW AFFINITY SORBENTS FOR PURIFICATION OF RECOMBINANT PROTEINS WITH THE USE OF CHITIN-BINDING DOMAIN AS AN AFFINITY TAG

被引：0
|
作者：
Kurek, Denis, V [1 ]
Lopatin, Sergey A. [1 ]
Tikhonov, Vladimir E. [2 ]
Varlamov, Valery P. [1 ]
机构：
[1] RAS, Ctr Bioengn, Moscow, Russia
[2] RAS, AN Nesmeyanov Inst Organoelement Cpds, Moscow, Russia
来源：
PROGRESS ON CHEMISTRY AND APPLICATION OF CHITIN AND ITS DERIVATIVES | 2010年 / 15卷
关键词：
chitin based sorbents; affinity chromatography; chitin-binding domain;
D O I：
暂无
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
With the increasing amounts of recombinant proteins production the problem of effective purification of produced proteins became one of major topics for research nowadays. Affinity chromatography proved to be the most dominant method for this aim and application of chitin based affinity sorbents in combination with use of chitin-binding domain as an affinity tag for purification of recombinant proteins showed its efficiency even in comparison with commercially available systems.
引用
收藏
页码：41 / 46
页数：6
相关论文
共 50 条
  • [1] Prospects of application of the chitin-binding domains to isolation and purification of recombinant proteins by affinity chromatography
    Kurek, D. V.
    Lopatin, S. A.
    Varlamov, V. P.
    APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2009, 45 (01) : 1 - 8
  • [2] Prospects of application of the chitin-binding domains to isolation and purification of recombinant proteins by affinity chromatography
    D. V. Kurek
    S. A. Lopatin
    V. P. Varlamov
    Applied Biochemistry and Microbiology, 2009, 45 : 1 - 8
  • [3] A new peptide-affinity tag for the detection and affinity purification of recombinant proteins with a monoclonal antibody
    Böldicke, T
    Struck, F
    Schaper, F
    Tegge, W
    Sobek, H
    Villbrandt, B
    Lankenau, P
    Böcher, M
    JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 240 (1-2) : 165 - 183
  • [4] Purification of recombinant proteins by chemical removal of the affinity tag
    Carole Rais-Beghdadi
    Mario A. Roggero
    Nicolas Fasel
    Christophe D. Reymond
    Applied Biochemistry and Biotechnology, 1998, 74 : 95 - 103
  • [5] Purification of recombinant proteins by chemical removal of the affinity tag
    Rais-Beghdadi, C
    Roggero, MA
    Fasel, N
    Reymond, CD
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1998, 74 (02) : 95 - 103
  • [6] Heparin-binding peptide as a novel affinity tag for purification of recombinant proteins
    Morris, Jacqueline
    Jayanthi, Srinivas
    Langston, Rebekah
    Daily, Anna
    Kight, Alicia
    McNabb, David S.
    Henry, Ralph
    Kumar, Thallapuranam Krishnaswamy Suresh
    PROTEIN EXPRESSION AND PURIFICATION, 2016, 126 : 93 - 103
  • [7] Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
    Arnau, Jos
    Lauritzen, Conni
    Petersen, Gitte E.
    Pedersen, John
    PROTEIN EXPRESSION AND PURIFICATION, 2006, 48 (01) : 1 - 13
  • [8] Expression and purification of recombinant cytoplasmic domain of human erythrocyte band 3 with hexahistidine tag or chitin-binding tag in Escherichia coli
    Ding, Y
    Jiang, WH
    Su, Y
    Zhou, HQ
    Zhang, ZH
    PROTEIN EXPRESSION AND PURIFICATION, 2004, 34 (02) : 167 - 175
  • [9] Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag
    Mohamed A. A. Abdelhamid
    Kei Motomura
    Takeshi Ikeda
    Takenori Ishida
    Ryuichi Hirota
    Akio Kuroda
    Applied Microbiology and Biotechnology, 2014, 98 : 5677 - 5684
  • [10] Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag
    Abdelhamid, Mohamed A. A.
    Motomura, Kei
    Ikeda, Takeshi
    Ishida, Takenori
    Hirota, Ryuichi
    Kuroda, Akio
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (12) : 5677 - 5684
12345