ISOELECTRICAL FOCUSING OF CONNECTIN BY AGAROSE-GEL ELECTROPHORESIS

被引：0
|
作者：
MATSUMURA, K
HORI, S
SUGIURA, H
SHIMIZU, T
机构：
[1] UNIV TOKYO,FAC MED,INST BRAIN RES,DEPT NEUROL,BUNKYO KU,TOKYO 113,JAPAN
[2] SHIMOSHIZU NATL HOSP,DEPT NEUROL,CHIBA 284,JAPAN
[3] TOKYO METROPOLITAN INST NEUROSCI,DEPT NEUROCHEM,FUCHU,TOKYO 183,JAPAN
来源：
JOURNAL OF BIOCHEMISTRY | 1990年 / 108卷 / 04期
关键词：
D O I：
10.1093/oxfordjournals.jbchem.a123232
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of ̃3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, following the two-dimensional gel electrophoresis, demonstrated that connectin was an acidic protein with an estimated pI of ̃5.7. © 1990 Copyright, 1990 by the Journal of Biochemistry.
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页码：513 / 515
页数：3
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