[3] Fritz Lipmann Inst, Leibniz Inst Age Res, D-07745 Jena, Germany
来源:
PHARMACEUTICALS
|
2013年
/
6卷
/
02期
关键词:
cell penetrating peptides;
CPPs;
formation of non-covalent complexes;
MPG peptides;
internalized amount of cargoes;
relationships between CPP;
cargo and cell type;
D O I:
10.3390/ph6020184
中图分类号:
R914 [药物化学];
学科分类号:
100701 ;
摘要:
Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as enzymes, antibodies, nucleotide binding proteins or substrates including nucleoside phosphates and enzyme inhibitors. Peptides, proteins and nucleotides are transported by fusing or conjugating them to cell penetrating peptides or by formation of non-covalent complexes. The latter is often preferred because of easy handling, uptake efficiency and auto-release of cargo into the live cell. In our studies complexes are formed with labeled or readily detectable cargoes for qualitative and quantitative estimation of their internalization. Properties and behavior of adhesion and suspension vertebrate cells as well as the protozoa Leishmania tarentolae are investigated with respect to proteolytic activity, uptake efficiency, intracellular localization and cytotoxicity. Our results show that peptide stability to membrane-bound, secreted or intracellular proteases varies between different CPPs and that the suitability of individual CPPs for a particular cargo in complex formation by non-covalent interactions requires detailed studies. Cells vary in their sensitivity to increasing concentrations of CPPs. Thus, most cells can be efficiently transduced with peptides, proteins and nucleotides with intracellular concentrations in the low micromole range. For each cargo, cell type and CPP the optimal conditions must be determined separately.
机构:
KIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, GermanyKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
Schober, Tim
Wehl, Ilona
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h-index: 0
机构:
KIT, Inst Funct Interfaces IFG, POB 3640, D-76021 Karlsruhe, GermanyKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
Wehl, Ilona
Afonin, Sergii
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h-index: 0
机构:
KIT, Inst Biol Interfaces IBG 2, POB 3640, D-76021 Karlsruhe, GermanyKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
Afonin, Sergii
Babii, Oleg
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h-index: 0
机构:
KIT, Inst Biol Interfaces IBG 2, POB 3640, D-76021 Karlsruhe, GermanyKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
Babii, Oleg
Iampolska, Anna
论文数: 0引用数: 0
h-index: 0
机构:
Taras Shevchenko Natl Univ Kyiv, Vul Volodymyrska 60, UA-01601 Kiev, Ukraine
Enamine Ltd, Vul Chervonotkatska 78, UA-02094 Kiev, UkraineKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
Iampolska, Anna
Schepers, Ute
论文数: 0引用数: 0
h-index: 0
机构:
KIT, Inst Funct Interfaces IFG, POB 3640, D-76021 Karlsruhe, GermanyKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
Schepers, Ute
Komarov, Igor, V
论文数: 0引用数: 0
h-index: 0
机构:
Taras Shevchenko Natl Univ Kyiv, Vul Volodymyrska 60, UA-01601 Kiev, Ukraine
Lumobiotics GmbH, Auerstr 2, D-76227 Karlsruhe, GermanyKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
Komarov, Igor, V
Ulrich, Anne S.
论文数: 0引用数: 0
h-index: 0
机构:
KIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
KIT, Inst Biol Interfaces IBG 2, POB 3640, D-76021 Karlsruhe, GermanyKIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany
机构:
Kobe Gakuin Univ, Fac Pharmaceut Sci, Lab Drug Delivery Syst, Chuo Ku, 1-1-3 Minatojima, Kobe, Hyogo 6508586, JapanKobe Gakuin Univ, Fac Pharmaceut Sci, Lab Drug Delivery Syst, Chuo Ku, 1-1-3 Minatojima, Kobe, Hyogo 6508586, Japan
Iwase, Yuko
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h-index:
机构:
Kamei, Noriyasu
Khafagy, El-Sayed
论文数: 0引用数: 0
h-index: 0
机构:
Kobe Gakuin Univ, Fac Pharmaceut Sci, Lab Drug Delivery Syst, Chuo Ku, 1-1-3 Minatojima, Kobe, Hyogo 6508586, Japan
Suez Canal Univ, Dept Pharmaceut & Ind Pharm, Fac Pharm, Ismailia 41522, EgyptKobe Gakuin Univ, Fac Pharmaceut Sci, Lab Drug Delivery Syst, Chuo Ku, 1-1-3 Minatojima, Kobe, Hyogo 6508586, Japan
Khafagy, El-Sayed
Miyamoto, Mitsuko
论文数: 0引用数: 0
h-index: 0
机构:
Toray Industries Ltd, Chuo Ku, 2-1-1 Nihonbashimuromachi, Tokyo 1038666, JapanKobe Gakuin Univ, Fac Pharmaceut Sci, Lab Drug Delivery Syst, Chuo Ku, 1-1-3 Minatojima, Kobe, Hyogo 6508586, Japan
机构:
INRA, Unite Rech Cidricoles Biotransformat Fruits & Leg, F-35653 Le Rheu, FranceINRA, Unite Rech Cidricoles Biotransformat Fruits & Leg, F-35653 Le Rheu, France
Le Bourvellec, C
Renard, CMGC
论文数: 0引用数: 0
h-index: 0
机构:
INRA, Unite Rech Cidricoles Biotransformat Fruits & Leg, F-35653 Le Rheu, FranceINRA, Unite Rech Cidricoles Biotransformat Fruits & Leg, F-35653 Le Rheu, France