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THE CONVERSION OF D-XYLOSE TO XYLITOL AND D-XYLONIC ACID BY A BOVINE LENS PREPARATION
被引:3
|作者:
LI, EY
FEATHER, MS
机构:
[1] Department of Biochemistry, University of Missouri, Columbia
基金:
美国国家卫生研究院;
美国国家科学基金会;
关键词:
D O I:
10.1080/07328309408009210
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Incubation of D-xylose with an aqueous solution of bovine lens protein (100 mg/mL) at 37 degrees C and pH. 7.0 gives both a reduced product (xylitol) and an oxidation product ( D-xylonic acid), which were both unequivocally identified by GLC (TMS ether derivative) and by GLC/MS. Studies of the reaction at a variety of conditions suggest that both the reduction and oxidation reactions are protein (possibly enzyme) catalyzed and appear to be unique to lens protein (albumin and ovalbumin do not catalyze the reaction).
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页码:499 / 505
页数:7
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