THERMOSTABLE, SALT-TOLERANT AMYLASE FROM BACILLUS-SP-64

被引：16
|
作者：
KHIRE, JM
PANT, A
机构：
[1] Division of Biochemical Sciences, National Chemical Laboratory, Pune
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 02期
关键词：
BACILLUS; SALT-TOLERANT AMYLASE; THERMOSTABLE AMYLASE;
D O I：
10.1007/BF01195840
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A thermostable, salt-tolerant amylase was produced by Bacillus sp. 64, with maximum amylase production (8.0 U/ml culture filtrate) after 24-h growth. Partially purified amylase was stable at 60-degrees-C for 30 min and 80% of the original activity was retained when incubated in 5 m NaCl over 24 h. Starch or dextrin was the best carbon source and peptone the best nitrogen source for the production of the enzyme. Amylase was secreted over a wide pH range (5 to 11) with the maximum activity between pH 7 and 8. Ca2+ and Mg2+ stimulated growth and enzyme production.
引用
收藏
页码：167 / 170
页数：4
相关论文
共 50 条
  • [11] Salt-tolerant and thermostable mechanisms of an endoglucanase from marine Aspergillus niger
    Li-Nian Cai
    Sheng-Nan Xu
    Tao Lu
    Dong-Qiang Lin
    Shan-Jing Yao
    Bioresources and Bioprocessing, 9
  • [12] Characterization of a salt-tolerant bacterium Bacillus sp from a membrane bioreactor for saline wastewater treatment
    Zhang, Xiaohui
    Gao, Jie
    Zhao, Fangbo
    Zhao, Yuanyuan
    Li, Zhanshuang
    JOURNAL OF ENVIRONMENTAL SCIENCES, 2014, 26 (06) : 1369 - 1374
  • [13] Phylogenetic characterization of a novel salt-tolerant Bacillus species: Description of Bacillus dipsosauri sp nov
    Lawson, PA
    Deutch, CE
    Collins, MD
    JOURNAL OF APPLIED BACTERIOLOGY, 1996, 81 (01): : 109 - 112
  • [14] Resolution Mechanism and Characterization of an Ammonium Chloride-Tolerant, High-Thermostable, and Salt-Tolerant Phenylalanine Dehydrogenase from Bacillus halodurans
    Wei Jiang
    Ya-Li Wang
    Bai-Shan Fang
    Applied Biochemistry and Biotechnology, 2018, 186 : 789 - 804
  • [15] Cryptococcus nodaensis sp nov, a yeast isolated from soil in Japan that produces a salt-tolerant and thermostable glutaminase
    Sato, I
    Kobayashi, H
    Hanya, Y
    Abe, K
    Murakami, S
    Scorzetti, G
    Fell, JW
    JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1999, 22 (03) : 127 - 132
  • [16] Cryptococcus nodaensis sp nov, a yeast isolated from soil in Japan that produces a salt-tolerant and thermostable glutaminase
    Sato, I.
    Kobayashi, H.
    Hanya, Y.
    Abe, K.
    Murakami, S.
    Scorzetti, G.
    Fell, J.W.
    Journal of Industrial Microbiology and Biotechnology, 1999, 22 (03): : 127 - 132
  • [17] Resolution Mechanism and Characterization of an Ammonium Chloride-Tolerant, High-Thermostable, and Salt-Tolerant Phenylalanine Dehydrogenase from Bacillus halodurans
    Jiang, Wei
    Wang, Ya-Li
    Fang, Bai-Shan
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2018, 186 (03) : 789 - 804
  • [18] Characterization of a salt-tolerant bacterium Bacillus sp. from a membrane bioreactor for saline wastewater treatment
    Xiaohui Zhang
    Jie Gao
    Fangbo Zhao
    Yuanyuan Zhao
    Zhanshuang Li
    Journal of Environmental Sciences, 2014, (06) : 1369 - 1374
  • [19] Characterization of a salt-tolerant bacterium Bacillus sp. from a membrane bioreactor for saline wastewater treatment
    Xiaohui Zhang
    Jie Gao
    Fangbo Zhao
    Yuanyuan Zhao
    Zhanshuang Li
    Journal of Environmental Sciences, 2014, 26 (06) : 1369 - 1374
  • [20] Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp SN5
    Bai, Wenqin
    Xue, Yanfen
    Zhou, Cheng
    Ma, Yanhe
    BIOTECHNOLOGY LETTERS, 2012, 34 (11) : 2093 - 2099
12345