NACL-DEPENDENT EXPRESSION OF AMILORIDE-BLOCKABLE NA+ CHANNEL IN XENOPUS OOCYTES

RNA was isolated from chicken lower intestine (both colon and coprodeum) and injected into Xenopus oocytes. Na-22+ fluxed measured after 1-4 days demonstrated the induction of an amiloride-blockable pathway. The Na+ transporter expressed by the exogenous RNA had a high affinity to amiloride (inhibitory constant < 0.1-mu-M), but was insensitive to ethylisopropyl amiloride, i.e., it is likely to be the apical Na+ channel. Functional channels were readily expressed in oocytes injected with RNA derived from chickens fed a low-NaCl diet. On the other hand, no channel activity was detected in oocytes injected with RNA isolated from chickens fed a high-NaCl diet. Thus the previously reported regulation of transport by the dietary NaCl intake involves modulations in the level of mRNA that codes either for the Na+ channel or a posttranscriptional regulator of the channel.