HIGH-LEVEL PRODUCTION OF BIOLOGICALLY-ACTIVE HUMAN CYTOSOLIC PHOSPHOLIPASE-A(2) IN BACULOVIRUS-INFECTED INSECT CELLS

被引：19
|
作者：
AMEGADZIE, BY
JIAMPETTI, D
CRAIG, RJ
APPELBAUM, E
SHATZMAN, AR
MAYER, RJ
DILELLA, AG
机构：
[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT MOLEC GENET,KING OF PRUSSIA,PA 19406
[2] SMITHKLINE BEECHAM PHARMACEUT,DEPT MED CHEM,KING OF PRUSSIA,PA 19406
来源：
GENE | 1993年 / 128卷 / 02期
关键词：
PHOSPHOLIPIDS; ARACHIDONIC ACID; CDNA; EICOSANOIDS; INFLAMMATION;
D O I：
10.1016/0378-1119(93)90580-V
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Infection of Spodoptera frugiperda insect cells with a recombinant baculovirus expressing human cytosolic phospholipase A2 (cPLA2) resulted in the production of biologically active protein. The level of recombinant human cPLA2 production in infected insect cells was at least 50-fold higher than that observed in human monoblast U937 cells.
引用
收藏
页码：307 / 308
页数：2
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