A Sensitive Fluorometric Method for Measurement of Vascular Permeability in Spinal Cord Injury

被引：8
|
作者：
Qu, Zhi-Xiang [1 ]
Xu, Jian [1 ]
Perot, Phanor L., Jr. [2 ]
Hogan, Edward L. [1 ]
机构：
[1] Med Univ S Carolina, Dept Neurol, Charleston, SC 29425 USA
[2] Med Univ S Carolina, Dept Neurosurg, Charleston, SC 29425 USA
来源：
JOURNAL OF NEUROTRAUMA | 1991年 / 8卷 / 02期
关键词：
D O I：
10.1089/neu.1991.8.149
中图分类号：
R4 [临床医学];
学科分类号：
1002 ; 100602 ;
摘要：
A sensitive fluorometric method was modified for the evaluation of drug action upon vascular permeability in rat spinal cord injury. Fluorescein isothiocyanate-conjugated dextran (FITC-D MW 71,200), used as a macromolecular tracer, was injected iv 2 hours before sacrifice. The optimal pH for FITC-D fluorescence was 8.2. The recovery in spinal cord was 101.4 +/- 4.0% (mean +/- SD). The extent of FITC-D extravasation, expressed as the vascular injury index (VII), was increased in proportion to the trauma force. The peak of VII after trauma was at 2 hours. This fluorometric method is sensitive, simple, and reliable for evaluation of drug effects upon vascular permeability in CNS trauma.
引用
收藏
页码：149 / 156
页数：8
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