ABSOLUTE QUANTIFICATION OF INTRACELLULAR NA+ USING TRIPLE-QUANTUM-FILTERED NA-23 NMR

被引：45
|
作者：
ALLIS, JL
SEYMOUR, AML
RADDA, GK
机构：
[1] JOHN RADCLIFFE HOSP,MRC,BIOCHEM & CLIN MAGNET RESONANCE UNIT,OXFORD OX3 9DU,ENGLAND
[2] UNIV OXFORD,DEPT BIOCHEM,OXFORD,ENGLAND
来源：
JOURNAL OF MAGNETIC RESONANCE | 1991年 / 93卷 / 01期
关键词：
D O I：
10.1016/0022-2364(91)90032-O
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a method for the absolute quantification of triple-quantum-filtered spectra of spin-3/2 nuclei. The method is illustrated by measuring the amount of intracellular Na+ in the perfused rat heart. There is good agreement between the result determined by the triple-quantum method and that measured using a simple single-quantum pulse-and-collect experiment. © 1991.
引用
收藏
页码：71 / 76
页数：6
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