HUMAN THYROID HORMONE-BETA-1 RECEPTOR PRODUCED BY RECOMBINANT BACULOVIRUS-INFECTED INSECT CELLS

被引：7

作者：

PUTLITZ, JZ

DATTA, S

MADISON, LD

MACCHIA, E

JAMESON, JL

机构：

[1] MASSACHUSETTS GEN HOSP,THYROID UNIT,BOSTON,MA 02114

[2] HARVARD UNIV,SCH MED,BOSTON,MA 02115

[3] INST ENDOCRINOL,PISA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81232-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thyroid hormone receptors are nuclear proteins which regulate transcription in a hormone dependent manner. The baculovirus expression system was used for the overexpression of the β1 isoform of the human thyroid hormone receptor. The baculovirus produced receptor binds tri-iodothyronine with high affinity, is specifically immunoprecipitated with a β1 specific antibody, and binds to DNA that contains a known thyroid hormone receptor recognition site. Large scale production and purification of baculovirus produced receptor will be useful for structure-function analyses and studies of transcriptional regulation. © 1991 Academic Press, Inc.

引用

页码：285 / 290

页数：6

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