HUMAN THYROID HORMONE-BETA-1 RECEPTOR PRODUCED BY RECOMBINANT BACULOVIRUS-INFECTED INSECT CELLS

被引：7
|
作者：
PUTLITZ, JZ
DATTA, S
MADISON, LD
MACCHIA, E
JAMESON, JL
机构：
[1] MASSACHUSETTS GEN HOSP,THYROID UNIT,BOSTON,MA 02114
[2] HARVARD UNIV,SCH MED,BOSTON,MA 02115
[3] INST ENDOCRINOL,PISA,ITALY
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 01期
关键词：
D O I：
10.1016/S0006-291X(05)81232-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thyroid hormone receptors are nuclear proteins which regulate transcription in a hormone dependent manner. The baculovirus expression system was used for the overexpression of the β1 isoform of the human thyroid hormone receptor. The baculovirus produced receptor binds tri-iodothyronine with high affinity, is specifically immunoprecipitated with a β1 specific antibody, and binds to DNA that contains a known thyroid hormone receptor recognition site. Large scale production and purification of baculovirus produced receptor will be useful for structure-function analyses and studies of transcriptional regulation. © 1991 Academic Press, Inc.
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收藏
页码：285 / 290
页数：6
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