THE EFFECT OF ASPARAGUS VIRUS-INFECTION ON ASPARAGUS TISSUE-CULTURE

被引：14
|
作者：
DEVRIESPATERSON, RM
EVANS, TA
STEPHENS, CT
机构：
[1] UNIV DELAWARE,CTR AGR RES & EDUC,DEPT PLANT & SOIL SCI,GEORGETOWN,DE 19947
[2] MICHIGAN STATE UNIV,DEPT BOT & PLANT PATHOL,E LANSING,MI 48824
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1992年 / 31卷 / 01期
关键词：
ASPARAGUS-OFFICINALIS; ASPARAGUS VIRUS-I; ASPARAGUS VIRUS-II; MICROPROPAGATION;
D O I：
10.1007/BF00043472
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The impact of asparagus virus I (AV-I), a potyvirus, and asparagus virus II (AV-II), an ilarvirus, on micropropagation of field-grown asparagus was studied. Apical shoot tips excised from singly or doubly-infected plants were slow to develop roots and had a 15 to 75% reduction in survival in culture, respectively, compared to those excised from virus-free plants. The four virus infection groups were ranked: virus-free > AV-II > AV-I > AV-I & II for capacity of explants to both root and survive in vitro. Micropropagated plants infected with AV-II exhibited slight reductions in fresh and dry weights, with greater reductions associated with infection with AV-I and double infection, compared to the virus-free controls. Eighty-one virus-infected apical shoot tips yielded 7 (8.6%) virus-free clones, as determined by rub inoculation on indicator plants.
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页码：31 / 35
页数：5
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