THE ARFLIKE GENE ENCODES AN ESSENTIAL GTP-BINDING PROTEIN IN DROSOPHILA

被引：135
|
作者：
TAMKUN, JW
KAHN, RA
KISSINGER, M
BRIZUELA, BJ
RULKA, C
SCOTT, MP
KENNISON, JA
机构：
[1] UNIV COLORADO,DEPT MOLEC CELLULAR & DEV BIOL,BOULDER,CO 80309
[2] NCI,DIV CANC TREATMENT,BIOL CHEM LAB,BETHESDA,MD 20892
[3] NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 08期
关键词：
D O I：
10.1073/pnas.88.8.3120
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have identified a Drosophila gene (arflike, arl) encoding a protein that is structurally related (almost-equal-to 55% identity) to the ADP-ribosylation factors (ARFs) of yeast and mammals. Biochemical analyses of purified recombinant arl-encoded protein revealed properties similar to the ARF proteins, including the ability to bind and hydrolyze GTP. Clear functional differences between arl and ARF proteins, including a complete lack of ARF activity, suggest that arl is not a functional homolog of ARF. A recessive lethal arl mutation was recovered, demonstrating that the arl locus is an essential gene. We conclude that the arl locus encodes an essential member of the ARF subfamily of small GTP-binding proteins in Drosophila.
引用
收藏
页码：3120 / 3124
页数：5
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