MAPPING OF THE HUMAN COL5A1 GENE TO CHROMOSOME 9Q34.3

被引:0
|
作者
CARIDI, G
PEZZOLO, A
BERTELLI, R
GIMELLI, G
DIDONATO, A
CANDIANO, G
GHIGGERI, GM
机构
[1] IST GIANNIAN GASLINI,DIV NEFROL,LARGO G GASLINI 5,I-16148 GENOA,ITALY
[2] IST GIANNIAN GASLINI,CITOGENET LAB,I-16148 GENOA,ITALY
[3] IST GIANNIAN GASLINI,SERV GENET MOLEC,I-16148 GENOA,ITALY
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中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A 353-bp region encoding for the NH2 terminus of the noncollagenic part of the alpha1(V) chain was amplified by the polymerase chain reaction (PCR), subcloned and sequenced. The subcloned PCR product (pGC1) presented the same nucleotide sequence as the original fragment from the published sequence of COL5A1. In situ hybridization, using pGC1 as a probe, mapped the COL5A1 gene to chromosome 9q34.3. This assignement shows that COL5A1 is not synthenic with COL5A2, which is localized together with other collagen genes on chromosome 2.
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页码:174 / 176
页数:3
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