Biosynthesis, Characterization, and Antimicrobial Activity of Silver Nanoparticles from Actinomycetes

Biosynthesis of silver nanoparticles using some Streptomyces species was reported, out of 46 strains tested, 15 strains showed ability to synthesized AgNPs. Among which the two potential Streptomyces M-13 and Streptomyces M-24 showed high potency of production of silver nanoparticles. Biosynthesized AgNPs from potent strain was characterized using UV-visible spectroscopy, FTIR analysis, Transmission Electron Microscope (TEM). TEM study indicated the formation of spherical shape distributed silver nanoparticles without aggregation varying from 10-20 nm in size. The silver nanoparticles synthesized by potential Streptomyces M-13 and Streptomyces M-24 showed good antibacterial activity against Gram-positive and Gram-negative bacteria by well diffusion method. The two potential Streptomyces species M-13 and M-24 was characterized and identified as Streptomyces graminofaciens and Streptomyces catenulae, respectively. The two Streptomyces strains reported in the present study are a newly added source for the biosynthesis of silver nanoparticles and these nanoparticles can be used potentially for biomedical application.