LIPASE-MEDIATED PRODUCTION OF ETHYL BUTYRATE AND BUTYL BUTYRATE IN NONAQUEOUS SYSTEMS

Lipases (EC 3.1.1.3) from Candida cylindracea, porcine pancreas, and Aspergillus niger (two types) were compared as catalysts for ethyl butyrate and butyl butyrate synthesis at 30 and 50°C using hexane as a reaction medium. Each enzyme had a distinct pH profile. The esterification catalysed by each lipase was inhibited by increasing the substrate concentration beyond a defined amount, but the concentration required varied from enzyme to enzyme and with the ester being formed. When the reaction temperature was raised from 30 to 50°C, ethyl butyrate synthesis was inhibited while butyl butyrate synthesis was enhanced with all enzymes tested. Overall, Candida cylindracea lipase produced the highest concentrations and yields of both esters. © 1990.