CRYSTALLIZATION OF A DELETION MUTANT OF THE R-SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE

被引：10
|
作者：
SU, Y
TAYLOR, SS
DOSTMANN, WRG
XUONG, NH
VARUGHESE, KI
机构：
[1] UNIV CALIF SAN DIEGO,DEPT BIOL,9500 GILMAN DR,LA JOLLA,CA 92093
[2] UNIV CALIF SAN DIEGO,DEPT CHEM,LA JOLLA,CA 92093
[3] UNIV CALIF SAN DIEGO,DEPT PHYS,LA JOLLA,CA 92093
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 03期
关键词：
REGULATORY SUBUNIT; CAMP DEPENDENT KINASE; CRYSTALLIZATION;
D O I：
10.1006/jmbi.1993.1224
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A single deletion (Δ 1 to 91) mutant of the regulatory subunit of the cAMP dependent protein kinase was crystallized. The crystals are hexagonal P6(1)22 (P6(5)22) with a = b = 88.7 Å and c = 179.9 Å. The crystals diffract to 3 Å resolution. There is one molecule per asymmetric unit. © 1993 Academic Press, Inc.
引用
收藏
页码：1091 / 1093
页数：3
相关论文
共 50 条
  • [41] DNA-MEDIATED GENE-TRANSFER OF A MUTANT REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
    ABRAHAM, I
    BRILL, S
    HYDE, J
    FLEISCHMANN, R
    CHAPMAN, M
    GOTTESMAN, MM
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1985, 260 (26) : 3934 - 3940
  • [42] STOICHIOMETRY OF CAMP IN ACTIVATION OF CAMP-DEPENDENT PROTEIN-KINASE
    WU, SM
    PADRON, M
    CHAU, V
    FEDERATION PROCEEDINGS, 1983, 42 (07) : 2255 - 2255
  • [43] PHOSPHORYLATION OF MAMMALIAN MYOSIN KINASES BY THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE OR CGMP-DEPENDENT PROTEIN-KINASE
    NISHIKAWA, M
    DELANEROLLE, P
    LINCOLN, TM
    ADELSTEIN, RS
    BIOPHYSICAL JOURNAL, 1983, 41 (02) : A152 - A152
  • [44] PHOSPHORYLATION OF THE REGULATORY SUBUNIT OF TYPE-II CAMP-DEPENDENT PROTEIN-KINASE BY CGMP-DEPENDENT PROTEIN-KINASE
    HUANG, LC
    CHARLTON, JP
    KING, L
    FEDERATION PROCEEDINGS, 1986, 45 (06) : 1551 - 1551
  • [45] REGULATION OF CAMP-DEPENDENT PROTEIN-KINASE BY THE PROTEIN-KINASE INHIBITOR PROTEINS
    UHLER, MD
    BAUDE, EJ
    OLSEN, SR
    GAMM, DM
    WEEKES, A
    REIMANN, EM
    DIGNAM, SS
    FASEB JOURNAL, 1994, 8 (07): : A1239 - A1239
  • [46] EFFECT OF THE THERMOSTABLE PROTEIN-KINASE INHIBITOR ON INTRACELLULAR-LOCALIZATION OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
    FANTOZZI, DA
    TAYLOR, SS
    HOWARD, PW
    MAURER, RA
    FERAMISCO, JR
    MEINKOTH, JL
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (24) : 16824 - 16828
  • [47] PHOSPHORYLATION OF RII SUBUNIT AND ATTENUATION OF CAMP-DEPENDENT PROTEIN-KINASE ACTIVITY BY PROLINE-DIRECTED PROTEIN-KINASE
    BRAUN, RK
    VULLIET, PR
    CARBONAROHALL, DA
    HALL, FL
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 289 (01) : 187 - 191
  • [48] CAMP-DEPENDENT PROTEIN-KINASE REGULATORY SUBUNIT PHOSPHORYLATION IN INTACT-CELLS
    MUMBY, MC
    SCOTT, CW
    METHODS IN ENZYMOLOGY, 1988, 159 : 139 - 146
  • [49] BIOCHEMICAL-PROPERTIES OF A MONOMERIC REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
    HERBERG, FW
    DOSTMANN, W
    TAYLOR, SS
    FASEB JOURNAL, 1993, 7 (07): : A1122 - A1122
  • [50] INJECTION OF CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE INTO ISOLATED CARDIAC MYOCYTES
    BRUM, G
    FLOCKERZI, V
    HOFMANN, F
    OSTERRIEDER, W
    TRAUTWEIN, W
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1983, 398 (02): : 147 - 154
12345