IDENTIFICATION OF BRUCELLA SPP BY USING THE POLYMERASE CHAIN-REACTION

被引：98
|
作者：
HERMAN, L
DERIDDER, H
机构：
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 06期
关键词：
D O I：
10.1128/AEM.58.6.2099-2101.1992
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The application of two synthetic oligonucleotides as probes and as primers in the polymerase chain reaction is presented for a specific, sensitive, and quick identification of Brucella spp. The specific oligonucleotide sequences were chosen on the basis of a 16S rRNA sequence alignment between Brucella abortus and Agrobacterium tumefaciens.
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页码：2099 / 2101
页数：3
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