EXTRACTION OF HIGH-QUALITY DNA FROM BLOODSTAINS USING DIATOMS

被引：9
|
作者：
GUNTHER, S [1 ]
HEROLD, J [1 ]
PATZELT, D [1 ]
机构：
[1] UNIV WURZBURG,INST VIROL,D-97078 WURZBURG,GERMANY
来源：
INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 1995年 / 108卷 / 03期
关键词：
DNA EXTRACTION; DIATOMS; PCR; STAIN ANALYSIS;
D O I：
10.1007/BF01844828
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
A simple method is described for the extraction of high quality DNA for PCR amplification. The DNA was extracted by using Chelex-100 ion exchange resin or a special cell lysis buffer containing proteinase K. For further purification the DNA was bound to silica in the presence of a chaotrophic agent. Hence it is possible to unlimitedly wash the bound DNA and inhibitory substances are removed. By using diatoms as a source of silicates, this method is very economical and can therefore be used as a routine method.
引用
收藏
页码：154 / 156
页数：3
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