BROMODEOXYURIDINE INCORPORATION INTO DNA OF HUMAN LEUKEMIA-CELLS IS NOT CONCENTRATION DEPENDENT

Leukemia blasts isolated from bone marrow aspirates of 44 adults with acute leukemia were incubated for 1 h with 0.008–32 μM bromodeoxyuridine (Brd‐Urd). After dual labeling with monoclonal anti‐BrdUrd antibodies and propidium iodide, the cells were analyzed by flow cytometry. Percent labeled cells and intensity of labeling were similar over concentrations of BrdUrd ranging from 0.8–32 μM—a 40‐fold range. Therefore, despite potential interpatient variability in nucleoside pharmacokinetics, commonly used doses of BrdUrd which are intended to achieve steady‐state plasma concentrations in the 8.0 μM range can be expected to provide a reliable estimate of the S‐phase fraction. Copyright © 1990 Wiley‐Liss, Inc.