CULTURE AND REGENERATION OF POPLAR MESOPHYLL PROTOPLASTS

被引：0

作者：

WANG, SP ^{[1
]}

XU, ZH ^{[1
]}

WEI, ZM ^{[1
]}

机构：

[1] SHANGHAI INST PLANT PHYSIOL,PLANT MOLEC GENET LAB,ACAD SINICA,SHANGHAI 200031,PEOPLES R CHINA

来源：

SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES | 1991年 / 34卷 / 05期

关键词：

POPULUS-TOMENTOSA; PROTOPLAST CULTURE; PLANT REGENERATION;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Populus tomentosa is one special species of poplar growing in North China. Mesophyll protoplasts were isolated from the axenic shoots and cultured in the modified KM8p liquid medium. Protoplast-derived cells started to divide after 7 days of culture. The frequency of cell division reached about 20% in 10 days. The yellowish green calli grew compact and nodular after the hormone concentration of medium was adjusted. Shoot formation occurred when the protoplast-derived calli were transferred onto MS medium containing zeatin and IAA or NAA. The shoots rooted readily on 1/2 MS hormone-free medium.

引用

页码：587 / 592

页数：6

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