Chromatographic fraction of extracts from the leaves and branches of Ouratea cuspidate (Ochnaceae) allowed the isolation and identificationof the mistures: of sitosterol and stigmasterol; two news maslinic acid ester derivative: 3-beta-Ostearoyl/palmitoyl-maslinic acid and 2-alpha-O-stearoyl/palmitoyl-maslinic acid, and lupeol, aand alpha-amyrin, besides the pure compounds: maslinic acid, amentoflavone and putraflavone, flavonoids 4', 5,7-trihydroxy-3', 5'-dimethoxy-flavone, 3,5,7,4'-tetrahydroxy-3'-methoxy-flavone, 5,7,4'-trihydroxy-3'-methoxy-3-O-beta-D-galactopyranosyl-flavone as well as the carbohydrate methyl-beta-D-glucopyranoside. The structures of compounds were deduced by spectral data analysis of NMR H-1, C-13 (HSQC, HMBC, COSY) besides ESI-MS/MS, GC/MS and comparison with literature data.