DETECTION OF HIV-1 RNA IN PLASMA BY ISOTHERMAL AMPLIFICATION (NASBA) IRRESPECTIVE OF THE STAGE OF HIV-1 INFECTION

被引：10
|
作者：
ZAAIJER, HL
KOK, W
TENVEEN, JH
REESINK, HW
FOOLEN, H
WINKEL, IN
HUISMAN, JG
CUYPERS, HTM
KIEVITS, T
LELIE, PN
机构：
[1] ORGANON TEKNIKA BV,5281 RM BOXTEL,NETHERLANDS
[2] PRINSENGRACHT HOSP,1017 JZ AMSTERDAM,NETHERLANDS
来源：
JOURNAL OF VIROLOGICAL METHODS | 1995年 / 52卷 / 1-2期
关键词：
HIV-1; RNA; AMPLIFICATION;
D O I：
10.1016/0166-0934(94)00160-I
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Using an experimental assay for isothermal amplification of HIV RNA in plasma (NASBA, Organon Teknika, Boxtel, The Netherlands), 70 samples from 59 HIV-1-infected persons and 29 samples from 28 uninfected volunteer blood donors were tested for the presence of HIV-1 RNA. The HIV-1-positive plasma samples were drawn from patients at various stages of infection: two samples from persons with signs of acute HIV infection (CDC stage I); 29 samples from 25 symptom-free persons (CDC stage II) and 39 samples from 32 persons with AIDS-related symptoms (CDC stage IV), All samples from HIV-1-infected persons had HIV-1 RNA, irrespective of the stage of infection (100% sensitivity). Testing the donor samples in duplicate, initially 54/58 samples (93%) tested negative for HIV-1 RNA. Repeated testing of the 4 samples with inconsistent test results showed all samples to be negative (specificity 100%). Detection of HIV-1 RNA in plasma by isothermal amplification (NASBA) promises to be a valuable alternative to the detection of HIV-1 RNA or DNA by the polymerase chain reaction.
引用
收藏
页码：175 / 181
页数：7
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