LIMITED PROTEOLYSIS AND SITE-DIRECTED MUTAGENESIS OF THE NF-KAPPA-B P50 DNA-BINDING SUBUNIT

被引：46
|
作者：
SODEOKA, M
LARSON, CJ
CHEN, L
LANE, WS
VERDINE, GL
机构：
[1] HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
[2] HARVARD UNIV,MICROCHEM FACIL,CAMBRIDGE,MA 02138
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1993年 / 3卷 / 06期
关键词：
D O I：
10.1016/S0960-894X(00)80294-2
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Tryptic digestion was used to obtain an active 41 kDa fragment of NF-kappaB. The major proteolysis products were comprised of residues 2-364 (p50(2-364)), 2-362 (p50(2-362)) and 2-366 (p50(2-366)). By site-directed truncation mutagenesis, overproducers for p50(2-364) and p50(2-366) were constructed. These vectors direct high level expression of homogeneous, active protein.
引用
收藏
页码：1095 / 1100
页数：6
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