OBSERVATION OF LARGE SUBUNIT PROTEIN COMPLEXES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：77
|
作者：
LOO, JA
机构：
[1] Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan, 48105
来源：
JOURNAL OF MASS SPECTROMETRY | 1995年 / 30卷 / 01期
关键词：
D O I：
10.1002/jms.1190300127
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Mass spectrometry with electrospray ionization and a magnetic sector instrument was used to detect multiply charged molecules for the non-covalently bound dimeric subunit protein complexes of horse liver alcohol dehydrogenase (M(r) similar to 80 000) and the tetrameric complexes of yeast alcohol dehydrogenase (M(r) similar to 147 000) and rabbit muscle pyruvate kinase (M(r) similar to 232 000). Ions for the pyruvate kinase complex represent one of the largest intact protein complexes resolved by mass spectrometry. Solvation of the large gas phase complexes is indicated by the mass spectrometric results.
引用
收藏
页码：180 / 183
页数：4
相关论文
共 50 条
  • [31] ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY AS A TOOL FOR MONITORING LIPID A HYDROLYSIS
    COLE, RB
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1995, 209 : 115 - ANYL
  • [32] ANALYSIS OF HYDROPHOBIC PROTEINS AND PEPTIDES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
    SCHINDLER, PA
    VANDORSSELAER, A
    FALICK, AM
    [J]. ANALYTICAL BIOCHEMISTRY, 1993, 213 (02) : 256 - 263
  • [33] ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY OF VITAMIN-D DERIVATIVES
    WILSON, SR
    TULCHINSKY, ML
    WU, YH
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1993, 3 (09) : 1805 - 1808
  • [34] ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY OF RECOMBINANTLY ENGINEERED ANTIBODY FRAGMENTS
    BOURELL, JH
    CLAUSER, KP
    KELLEY, R
    CARTER, P
    STULT, JT
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (13) : 2088 - 2095
  • [35] DETECTION OF METHOXYLATED ANIONS OF FULLERENES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
    WILSON, SR
    WU, YH
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (22) : 10334 - 10337
  • [36] WEIGHING THE EVIDENCE FOR STRUCTURE - ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY OF PROTEINS
    ROBINSON, CV
    RADFORD, SE
    [J]. STRUCTURE, 1995, 3 (09) : 861 - 865
  • [37] ONLINE CAPILLARY ELECTROPHORESIS ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY OF NUCLEOTIDES
    ZHAO, ZX
    WAHL, JH
    UDSETH, HR
    HOFSTADLER, SA
    FUCIARELLI, AF
    SMITH, RD
    [J]. ELECTROPHORESIS, 1995, 16 (03) : 389 - 395
  • [38] CHARACTERIZATION OF HAPTEN BINDING TO IMMUNOCONJUGATES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
    STRAUB, KM
    LEVY, MJ
    [J]. BIOCONJUGATE CHEMISTRY, 1994, 5 (03) : 194 - 198
  • [39] CONFORMATIONAL EFFECTS IN PROTEIN ELECTROSPRAY-IONIZATION MASS SPECTROMETRY
    Li, Jinyu
    Santambrogio, Carlo
    Brocca, Stefania
    Rossetti, Giulia
    Carloni, Paolo
    Grandori, Rita
    [J]. MASS SPECTROMETRY REVIEWS, 2016, 35 (01) : 111 - 122
  • [40] OBSERVATION AND IMPLICATIONS OF HIGH MASS-TO-CHARGE RATIO IONS FROM ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
    WINGER, BE
    LIGHTWAHL, KJ
    LOO, RRO
    UDSETH, HR
    SMITH, RD
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1993, 4 (07) : 536 - 545
12345