Proteomic analysis of the plasma samples of patients with stable angina pectoris

Objective: In this study, the results of the proteomic analysis of plasma samples of stable angina patients are presented. Background: Plasma samples from 44 patients were analysed in two groups: (A) a control group of 22 patients without significant coronary artery disease and (B) 22 patients admitted with stable angina pectoris. Methods: During coronary angiography, plasma samples were collected and further analysed using two-dimensional gel electrophoresis, image analysis and mass spectrometry. Results: Five spots were found with significantly increased or decreased normalised volumes resulting from either a change in the amount of protein or changes in posttranslational modifications. Spots containing serum amyloid A protein and fibrinogen gamma chains displayed increased normalised volumes, and spots containing apoliprotein A-1 and pigment epithelium derived factor showed decreased normalised volumes. Conclusions: Almost identical proteomic profiles of the stable angina pectoris group compared with the control group corresponded well with clinical results comparing stable patients with the general population. (C)2012 The Czech Society of Cardiology. Published by Elsevier Urban & Partner Sp.z.o.o. All rights reserved.