THIOREDOXIN REDUCTION DEPENDENT ON ALPHA-KETOACID OXIDATION BY ALPHA-KETOACID DEHYDROGENASE COMPLEXES

被引：36
|
作者：
BUNIK, V [1 ]
FOLLMANN, H [1 ]
机构：
[1] UNIV KASSEL, FACHBEREICH BIOL CHEM, D-34109 KASSEL, GERMANY
来源：
FEBS LETTERS | 1993年 / 336卷 / 02期
关键词：
THIOREDOXIN; THIOREDOXIN REDUCTASE; ALPHA-KETOACID DEHYDROGENASE COMPLEX; INSULIN; LIPOATE; MITOCHONDRION;
D O I：
10.1016/0014-5793(93)80801-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The pyruvate and alpha-ketoglutarate dehydrogenase complexes isolated from pig heart mitochondria promote the reduction of thioredoxin in the presence of their alpha-ketoacid substrates, coenzyme A, and free lipoate. Substrate-specific generation of reduced thioredoxin was established by two independent methods, viz. reduction of insulin and thioredoxin reductase-catalyzed NADPH formation. Dihydrolipoate accumulating in the absence of NAD+ is the likely intermediate. A redox function in alpha-ketoacid oxidation provides a potential role for the specific thioredoxins previously identified by us in mitochondria.
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页码：197 / 200
页数：4
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