Biosurfactant Production by Pseudomonas aeruginosa Strains on 3ml of inoculum size

Aim: To produce biosurfactants from Pseudomonas aeruginosa using agricultural resource and to produce Biosurfactants using low cost materials. Study design: Descriptive study Place and duration of study: Study was conducted at Institute of Molecular Biology and Biotechnology in University of Lahore. Duration of the study was two years. Methods: The volume of sample taken are 3ml, of innoculum from growing culture of Pseudomonas aeruginosa was isolated from contaminated soil collected from industrial area of District Kasoor and flasks were then placed into an orbital shaker at speed of 120rpm. The samples were collected in sterile screw capped bottle, 4-5cm deep from soil surface aseptically. Samples were stored at 4 degrees C till further use. After every 24h, culture broth from each flask was taken to estimate bacterial cell mass. Methods: The volume of sample taken are 3ml, of innoculum from growing culture of Pseudomonas aeruginosa was isolated from contaminated soil collected from industrial area of District Kasoor and flasks were then placed into an orbital shaker at speed of 120rpm. The samples were collected in sterile screw capped bottle, 4-5cm deep from soil surface aseptically. Samples were stored at 4 degrees C till further use. After every 24h, culture broth from each flask was taken to estimate bacterial cell mass. Results: Surface tension was 66.1, 63.2, 48.2 and 45.7 mN/m at time 24, 48, 72 and 96 hours respectively at constant temperature of 37 degrees C and molasses used 0.25g with 3ml inoculum size. The rhamnolipid production was 0.23, 0.82, 1.75 and 1.98g/L respectively. Similarly the bacterial cell mass was 0.2, 0.18, 0.22 and 0.4 g/L respectively. Conclusion: After optimizing various growth and environmental factors a production of rhamnolipid was achieved.