MOLECULAR CHARACTERIZATION OF THE NODULATION GENE, NODT, FROM 2 BIOVARS OF RHIZOBIUM-LEGUMINOSARUM

被引：43
|
作者：
SURIN, BP
WATSON, JM
HAMILTON, WDO
ECONOMOU, A
DOWNIE, JA
机构：
[1] CSIRO, DIV PLANT IND, GPO BOX 1600, CANBERRA, ACT 2601, AUSTRALIA
[2] JOHN INNES INST, NORWICH NR4 7UH, NORFOLK, ENGLAND
[3] AGR GENET CO, AFRC, INST PLANT SCI RES, CAMBRIDGE LAB, CAMBRIDGE CB2 2JB, ENGLAND
[4] AFRC, INST PLANT SCI RES, NORWICH NR4 7UH, ENGLAND
来源：
MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 02期
关键词：
D O I：
10.1111/j.1365-2958.1990.tb00591.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA sequencing of the nodlJ region from Rhizobium leguminosarum biovar trifolii revealed the nodT gene immediately downstream of nodJ. DNA hybridizations using a nodT‐specific probe showed that nodT is present in several R. leguminosarum strains. Interestingly, a flavonoid‐inducible nodT gene homologue in R. leguminosarum bv. viciae is not in the nodABCIJ operon but is located downstream of nodMN. The sequence of the nodT gene from bv. viciae was determined and a comparison of the predicted aminoacid sequences of the two nodT genes shows them to be conserved; the predicted protein sequences appear to have a potential transit sequence typical of outer‐membrane proteins. Mutations affecting nodT in either biovar had no observed effect on nodulation of the legumes tested. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：245 / 252
页数：8
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