Production and optimization of L-asparaginase in Escherichia coli

被引：0

作者：

Mohamed, Zeinat K. ^{[1
]}

Elnagdy, Sh. M. ^{[1
]}

Seufi, A. E. ^{[2
]}

Gamal, M. ^{[1
]}

机构：

[1] Cairo Univ, Fac Sci, Bot & Microbiol Dept, Giza, Egypt

[2] Cairo Univ, Fac Sci, Entomol Dept, Giza, Egypt

来源：

EGYPTIAN JOURNAL OF BOTANY | 2016年 / 56卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

LASPARAGINASE (L-ASNase) has been widely used as a therapeutic agent in the treatment for various lymphoblastic leukemia diseases. This study aimed to isolate and purify local bacterial isolates that are capable of producing L-ASNase, so 150 bacterial isolates from the Nile River where isolated, purified and their ability to produce L-ASNase was assessed. Among these isolates, 32 bacterial isolates showed their ability to produce L-ASNase, so they were selected for further studies. The most active bacterial isolate in the production of L-ASNase was selected, identified as Escherichia coli and named MG27. Enzyme localization was determined in cultures grown under aerobic and anaerobic conditions. E. coli MG27 was found to produce more L-ASNase in anaerobic conditions compared with that produced under aerobic conditions with 128 folds. Finally, in an attempt to determine the optimum conditions for periplasmic L-ASNase production, the influence of several cultural factors was investigated.

引用

页码：203 / 224

页数：22

共 50 条

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