THIOPHILIC INTERACTION CHROMATOGRAPHY OF SWEET-POTATO BETA-AMYLASE

被引：8
|
作者：
FRANCOFRAGUAS, L [1 ]
BATISTAVIERA, F [1 ]
机构：
[1] FAC QUIM MONTEVIDEO,CATEDRA BIOQUIM,GRAL FLORES 2124,CASILLA CORREOS 1157,MONTEVIDEO,URUGUAY
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 604卷 / 01期
关键词：
D O I：
10.1016/0021-9673(92)85534-Z
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The affinity of sweet potato beta-amylase toward two kinds of thiophilic adsorbents [the so-called thiophilic or T-gel and 3-(2-pyridylsulphido)-2-hydroxypropylagarose or PyS-gel] was demonstrated. Enzyme adsorption on both gels was promoted by antichaotropic salts. Lower salt requirements and higher protein recoveries were observed for the PyS gel, even though its ligand concentration was half that of T-gel. For the former gel, the effectiveness series for sulphates was Na2SO4 > (NH4)2SO4 > K2SO4 > MgSO4. Based on the thiophilic character exhibited by the pure sweet potato beta-amylase and the reversibility of the salt effects, a new method for its purification from crude extracts was developed.
引用
收藏
页码：103 / 107
页数：5
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